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Additional studies on holoenzyme containing 4 mol of AACK per mol of holoenzyme showed that a given amount of the beta subunit also inhibited the holoenzyme. This inhibition could be overcome with an excess of holoenzyme. Other studies demonstrated that the beta subunit inhibited the acylation of holoenzyme.

beta Subunit was found to bind 2 mol of AACK per mol of holoenzyme. At a ratio of 1:30, the concentration of the beta subunit was used to titrate the AACK activity. The stoichiometry of interaction between AACK and the beta subunit was determined by using various concentrations of both proteins. Interactions of elongator acetyl-CoA carboxylase (AACK), the biotin protein that is the prosthetic group of the enzyme and the beta subunit of carboxylase were studied. Download Euro Truck Simulator 2.Interaction of elongator acetyl-CoA carboxylase with the beta subunit of rat liver biotin-containing carboxylase. Euro Truck Simulator 2 Road to the Black Sea Crack.

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